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Peptide reconstitution calculator

Get the exact units to draw on a U-100 insulin syringe. Enter the peptide in the vial, the water you added and your dose.

Updated October 29, 2026

Bacteriostatic water you added to the vial.
Draw to–
Volume–
Concentration–
Doses in the vial–

Units are tick marks on a U-100 insulin syringe: 100 units = 1 mL.

How the units math works

A U-100 insulin syringe holds 100 units in 1 mL, so each unit is 0.01 mL. Once you know how much peptide is in each mL, the units follow:

concentration (mg/mL) = peptide in vial (mg) ÷ water added (mL)
volume per dose (mL) = dose (mg) ÷ concentration
units to draw = volume per dose × 100

Example: 5 mg mixed with 2 mL of water is 2.5 mg/mL. A 250 mcg (0.25 mg) dose is 0.1 mL, which is 10 units. The vial holds 20 doses.

Picking how much water to add

More water makes each dose a bigger, easier-to-read draw; less water keeps small syringes usable. A common goal is a dose that lands on a whole tick between about 5 and 50 units. Change the water amount above and watch where the dose lands. The water doesn't change how many doses the vial holds, only how many units each one is.

New to mixing? Follow the step-by-step guide with pictures. Want the cost of each of those doses? Use the peptide cost calculator.

Questions

How many units do I draw on an insulin syringe?

Divide the peptide in the vial by the water you added to get mg per mL. Divide your dose by that to get mL, then multiply by 100 for units on a U-100 syringe. 5 mg in 2 mL is 2.5 mg/mL; a 0.25 mg dose is 0.1 mL, or 10 units.

How much bacteriostatic water should I add?

Any amount that makes your dose an easy, readable draw on your syringe. More water means more units per dose. The amount of water doesn't change how many doses the vial holds.

What's the difference between mg and mcg?

1 mg = 1,000 mcg. Mixing them up is the most common dosing mistake, so the calculator lets you pick the unit for the vial and the dose separately.

Do I need to recalculate when my dose changes?

Yes. Every dose change changes the units. PepKeep keeps step-up plans on schedule and shows the units to draw at each reminder.

Is this calculator medical advice?

No. It does the arithmetic on the numbers you enter. It doesn't recommend compounds or doses, and you should double-check every draw.

Let PepKeep do this math for you, every day.

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More tools

Peptide cost calculatorCost per shot, week, month and yearWhat a stack costsWork out your monthly spendTracker apps comparedPepKeep vs Peptide Tracker, Regimen and moreStep-by-step mixing guideMix a vial and draw a dose, with picturesUnits charts by peptideUnits to draw for common vial sizes and water amounts

Sources

This page only does the math. The FDA has warned that confusion between milliliters, milligrams and units can lead to dosing errors, so check your syringe markings too.