NAD+ reconstitution chart

How many units to draw for NAD+ on a U-100 insulin syringe, for every common vial size and water amount.

Updated October 29, 2026

These charts are arithmetic for a U-100 insulin syringe (1 unit = 0.01 mL), not dose recommendations. Use the dose from your own protocol, and double-check every draw.

Quick answer

A NAD+ 1000 mg vial mixed with 4 mL of bacteriostatic water is 250 mg per mL. Every unit on a U-100 syringe is 0.01 mL, so 10 units = 25 mg and 100 mg = 40 units. Change the water and every number changes, which is why "how many units" only makes sense next to the vial size and the water.

NAD+ units charts

NAD+ 500 mg vial

Water added	Strength	5 units =	10 units =	25 units =	100 mg =	Whole vial	Calculator
2 mL	250 mg/mL	12.5 mg	25 mg	62.5 mg	40 units	200 units	Open
4 mL	125 mg/mL	6.25 mg	12.5 mg	31.25 mg	80 units	400 units	Open
5 mL	100 mg/mL	5 mg	10 mg	25 mg	100 units	500 units	Open

NAD+ 1000 mg vial

Water added	Strength	5 units =	10 units =	25 units =	100 mg =	Whole vial	Calculator
2 mL	500 mg/mL	25 mg	50 mg	125 mg	20 units	200 units	Open
4 mL	250 mg/mL	12.5 mg	25 mg	62.5 mg	40 units	400 units	Open
5 mL	200 mg/mL	10 mg	20 mg	50 mg	50 units	500 units	Open

Different numbers? The reconstitution calculator works for any vial, water and dose, and shows the mark on a syringe. New to mixing? See the step-by-step guide with pictures.

How to read the chart

strength (mg per mL) = mg in vial ÷ mL of water
mg in a draw = units ÷ 100 × strength
units for a dose = dose (mg) ÷ strength × 100

More water makes each unit weaker, so the same dose takes more units and is easier to measure. The water never changes how much NAD+ is in the vial, only how many units each dose is.

Track your NAD+ vials

Enter your vial and water once and PepKeep does this math at every dose: the reminder shows the exact units to draw, each logged dose comes out of the vial, and you see how many doses are left, the day it runs out, and what each shot costs. It works for step-up plans and on/off cycles too, on iPhone, Android and desktop. Try it free for 7 days, no card needed.

Questions

How many units is 100 mg of NAD+ in a 1000 mg vial with 4 mL of water?

40 units on a U-100 syringe. 1000 mg ÷ 4 mL = 250 mg per mL, and 100 mg ÷ 250 mg per mL = 0.4 mL, which is 40 units.

How much bacteriostatic water should I add to NAD+?

Any amount that makes your dose easy to read on your syringe. More water means more units per dose; it never changes how many mg are in the vial. Pick a water amount, then use the chart or calculator to see where your dose lands.

Does the syringe size change the units?

No. On every U-100 insulin syringe, 1 unit is 0.01 mL. A 0.3 mL or 0.5 mL syringe just holds fewer units; each unit is the same size.

Is this a dosing guide?

No. It converts mg, mL and units. It doesn't recommend a dose; use the one from your own protocol and clinician.

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Sources

Peptide facts come from these sources. The chart itself is arithmetic.